Meeting Abstract

87.2  Friday, Jan. 6  Toward an understanding of the molecular mechanisms of barnacle larval settlement: a comparative transcriptomic approach CHEN, Z-F*; MATSUMURA, K; WANG, H; ARELLANO, S.M.; YAN, X; ALAM, I; ARCHER, J.A.C.; BAJIC, V.B.; QIAN, P-Y; The Hong Kong Univ. of Science and Technology (HKUST); HKUST; HKUST; Woods Hole Oceanographic Institution; HKUST; King Abdullah Uni. of Science and Technology (KAUST); KAUST; KAUST; HKUST chenzf@ust.hk

The barnacle, Balanus amphitrite, is a globally distributed biofouler and a model species in intertidal ecology and larval settlement studies. However, a lack of genomic information has hindered the comprehensive elucidation of the molecular mechanisms coordinating its larval settlement. We used 454 pyrosequencing-based transcriptomic profiling to identify key molecular changes during larval settlement. We collected 630,845 reads of which 215,308 were from the larval stages and 415,537 from the adults; 23,451 contigs were generated while 77,785 remained as singletons. We annotated 31,720 of the 92,322 predicted open reading frames, which matched hits in the NCBI NR database, and identified 7,954 putative genes that were differentially expressed between the larval and adult stages. Of these, several genes were further characterized with quantitative real-time PCR and in situ hybridization, revealing some key findings: 1) vitellogenin was uniquely expressed in late nauplius stage, suggesting it may be an energy source for the subsequent non-feeding cyprid stage; 2) the locations of mannose receptor-like genes suggested they may be involved in the sensory system of cyprids; 3) 20 kDa-cement protein homologues were expressed in the cyprid cement gland and probably function during attachment; and 4) receptor tyrosine kinases were expressed higher in cyprid stage and may be involved in signal perception. Overall, our results provide not only the basis of several new hypotheses about gene functions during larval settlement, but also the availability of the transcriptome dataset for B. amphitrite for further exploration of larval settlement and developmental pathways.